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. 2019 Apr 24;13:164. doi: 10.3389/fncel.2019.00164

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Copyright © 2019 Lenoir, Varangot, Lebouvier, Galli, Hommet and Vivien.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of tPA-HaloTag® plasmids. (A) Timeline of the experiments. Representative confocal images of cortical and hippocampal neurons transfected (+) or not (–) at DIV12 (Day In Vitro) with pSyn_tPA-HaloTag® and exposed 48 h later (+) or not (–) to the HaloTag® TMR ligand, together with an immunocytochemistries (ICC) against tPA (blue) and MAP2 (green). Scale Bar: 20 μm. (B) Transfection of cortical neurons at DIV 12 with pSyn_tPA-HaloTag® (red) and pCMV_GFP (green) plasmids, exposed 4 h later to the HaloTag® TMR ligand and ICC performed to reveal tPA (in blue). Z-stack and scale Bar: 50 μm (whole neuron) and 10 μm (1 and 2: whole neuron zoom). (C) Timeline of the experiments. Transfections of pCMV_tPA-HaloTag® or pSyn_tPA-HaloTag® (both revealed in red) and immunochemistry raised against MAP2 (in blue) show similar neuronal expressions of tPA-HaloTag® with the two constructs. Scale of tPA-HaloTag® with the two constructs. Scale Bar: 50 μm (whole neuron).