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. 2019 Apr 24;10:879. doi: 10.3389/fimmu.2019.00879

Figure 3.

Figure 3

Characteristics of expanded NK cells on day 14 in K562 and K562-OX40L group. (A) The cytotoxicity of expanded NK cells toward K562 cells and (B) ADCC of expanded NK cells against rituximab-coated Raji cells were measured by CFSE-based flow cytometry assay at effector-to-target (E:T) ratio of 2:1, 1:1, 0.5:1 for 4 h. Results presented are the mean of 6 donors and error bars represent the mean ± SD. (C) Expression of surface receptors on NK cells were assayed for CD16, NKG2A, NKG2C, CD57, CD8a, NKp30, NKp46, CD62L, NKG2D, and DNAM-1. Representative plots are shown in NK cell subset from the same donor, K562 group (red), and K562-OX40L group (green), NK cells on day 0 before co-culture (blue).