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. 2019 Apr 24;10:879. doi: 10.3389/fimmu.2019.00879

Figure 4.

Figure 4

Irradiated K562-OX40L with short exposure to different concentration of IL-21 results in pronounced expansion of NK cells for 5 weeks. PBMCs were co-cultured with γ-irradiated K562 (red) or K562-OX40L (green) with 10 U/mL of IL2 and 5 ng/ml (A), 50 ng/ml (B), 100 ng/ml (C) of IL-21 at day 0 and re-stimulated at day 7, 14 with 100 U/mL of IL-2 and 5 ng/mL of IL-15. The medium was replaced every 2–3 days. (D) Each of IL-21 added groups was combined and compared within K562 and K562-OX40L groups (K562, n = 7; and K562-OX40L, n = 10; error bars, mean ± SD; *p < 0.05; **p < 0.01; ***p < 0.001).