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. 2019 Apr 24;10:864. doi: 10.3389/fmicb.2019.00864

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Copyright © 2019 Hartmann, Piontkivska, Moreira and Silva Pereira.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Expression analysis of glucosylceramide pathway genes after 4 h of exposure to 1-decyl-3-methylimidazolium chloride ([C₁₀mim]Cl, black bars), compared to the control (white bars). Axis y represents the fold-change relative to time-zero (^∗∗p < 0.01). (B) Growth assessment in solid media supplemented with 0.05, 0.075, or 0.1 mM of [C₁₀mim]Cl. 10⁵, 10⁴, and 10³ conidia were inoculated in each plate. (C) Sphingoid bases accumulation profile of glucosylceramide pathway mutants and parental strain (wt) after 4 h of exposure to [C₁₀mim]Cl. The peaks assigned to phytosphingosine, sphingosine and dihydrosphingosine were confirmed by their standards. The peak marked with an asterisk likely corresponds to 9-methyl-4,8-sphingadiene (more details in Supplementary Figure S5).