Table 3.
Gene expression analysis (qRT-PCR) of genes involved in the synthesis of chitin, α-glucans and β-glucans after exposure to dodecyltributylphosphonium chloride ([P4 4 4 12]Cl), 1-decyl-3-methylimidazolium chloride ([C10mim]Cl) or cholinium decanoate for 4 h.
| Gene | Control | [P44412]Cl | [C10mim]Cl | Cholinium decanoate |
|---|---|---|---|---|
| chsA | 0.97 | 1.37 | 0.76 | 1.03 |
| chsB | 1.14 | 3.01 | 1.71 | 1.73 |
| chsC | 0.90 | 0.46 | 0.48 | 1.18 |
| chsD | 1.08 | 3.22 | 1.60 | 1.33 |
| chsF | 1.04 | 0.56 | 0.86 | 0.95 |
| chsG | 1.02 | 1.11 | 1.04 | 0.89 |
| csmA | 0.92 | 3.11 | 1.16 | 1.72 |
| csmB | 0.98 | 1.24 | 1.04 | 1.21 |
| agsA | 0.86 | 2.04 | 0.91 | 1.13 |
| agsB | 0.91 | 1.33 | 1.03 | 1.29 |
| fksA | 1.06 | 6.58 | 1.56 | 1.65 |
Values represent the fold-change relative to time-zero followed by their standard deviation. Four biological replicates were performed. Values in bold are statistically significant compared to the control (p < 0.05).