Table 1.
Bacterial strains and plasmids used and constructed in this study.
| Strain or plasmid | Descriptiona | References/Source |
|---|---|---|
| Strains P. damselae subsp. damselae | ||
| RM-71 | Isolated from turbot; carries pPHDD1 plasmid | Fouz et al., 1992 |
| MT151 | RM-71ΔrstB | Terceti et al., 2017 |
| MT319 | RM-71ΔrstA | This study |
| MT157 | MT151 with pRstAB (complemented mutant); CmR | Terceti et al., 2017 |
| MT245 | MT319 with pRstAB (complemented mutant); CmR | This study |
| LD-07 | Isolated from gilthead seabream; does not carry pPHDD1plasmid | Vera et al., 1991 |
| MT341 | LD-07ΔrstA | This study |
| MT340 | LD-07ΔrstB | This study |
| MT341C | MT341 with pRstAB (complemented mutant); CmR | This study |
| MT340C | MT340 with pRstAB (complemented mutant); CmR | This study |
| AR217 | RM-71ΔepsL | Rivas et al., 2015b |
| AR129 | RM-71ΔhlyAch | Rivas et al., 2013b |
| AR133 | RM-71ΔhlyApl | Rivas et al., 2011 |
| AR64 | RM-71Δdly | Rivas et al., 2011 |
| AR158 | RM-71ΔhlyAplΔhlyAch | Rivas et al., 2013b |
| AR119 | RM-71ΔdlyΔhlyAch | Rivas et al., 2013b |
| AR78 | RM-71ΔdlyΔhlyApl | Rivas et al., 2011 |
| AR89 (3Δ) | RM-71ΔdlyΔhlyAplΔhlyAch | Rivas et al., 2013b |
| E. coli | ||
| DH5α | Cloning strain | Laboratory stock |
| S17-1-λpir | RP4-2 (Km::Tn7, Tc::Mu-1) pro-82 λpir recA1 endA1 thiE1 hsdR17 creC510 | Herrero et al., 1990 |
| β-3914 | F- RP4-2-Tc::Mu ΔdapA::(erm-pir) gyrA462 zei-298::Tn10 (KmR EmR TcR) | Le Roux et al., 2007 |
| Plasmids | ||
| pMRB24 | Cloning vector, mob, CmR | Le Roux et al., 2011 |
| pRstAB | pMRB24 with rstAB genes; CmR | Terceti et al., 2017 |
| pNidkan | Suicide vector derived from pCVD442: KmR | Mouriño et al., 2004 |
aCmR, chloramphenicol resistance; KmR, kanamycin resistance: EmR, erythromycin resistance; TcR, tetracycline resistance; AmpR, ampicillin resistance; Δ, gene deletion.