Fig. 2.

Ability of HIV-1-specific CD8⁺ T cells primed from naïve cells to suppress HIV-1 replication.

(a) Ability of RF10-specific T cell lines primed with LPS or 3′3′-cGAMP to suppress the replication of HIV-1. NL4–3-infected CD4⁺ T cells from an HLA-A*24:02⁺ donor were co-cultured with T cell lines at different E:T ratios. Relative viral suppression ratio was calculated as follows: percent inhibition of primed T cell lines/that of control clone. The results at the E:T ratio of 1:1 are shown. (b) Comparison of relative viral suppression ability between LPS-primed T cell lines and 3′3′cGAMP-primed ones from each individual. Data are shown as the means and SD of triplicate assays. Statistical analysis was conducted by use of the non-parametric Mann Whitney test (a), a paired t-test (b). Horizontal bars indicate median values (a). *P < .05, **P < .01. TNI, T cells were not induced.