Table 1.

Summary of flow cytometer optical configurations, parameters measured and gating strategy

Cytometer	Laser wavelength, Power	Detectors	DNA dyes	Parameters measured	Gating strategy (to exclude clumps and debris)
				(A = Area, H = Height, W = Width)
Mo‐Flo legacy	MULTILINE UV (330–360 nm), 300 mW	BP 447/60	DAPI, HO	DAPI (H) HO (H)	FSC (H) versus Pulse width
	457 nm, 300 mW	LP 490	CA3	CA3 (H)
	488 nm, 200 mW	LP 620	PI	PI (H)
BD Influx	355 nm, 100 mW	BP 460/50	DAPI, HO	DAPI (H) HO (H)	DAPI (H) versus Trigger pulse width
	405 nm, 100 mW	BP 447/60	DAPI, HO	DAPI (H) HO (H)
	488 nm, 200 mW	LP 620	PI	PI (H)
	561 nm, 100 mW	BP 610/20	PI	PI (H)
BD LSRll	355 nm, 20 mW	BP 450/50	DAPI, HO	DAPI (A) HO (A)	DAPI (A) versus DAPI (W) HO (A) versus HO (W)
	405 nm, 25 mW	BP 450/50	DAPI, HO	DAPI (A) HO (A)
	488 nm, 20 mW	BP 610/20	PI	PI (A)
BD LSRFortessa	355 nm, 20 mW	BP 450/50	DAPI, HO	DAPI (A) HO (A)	DAPI (A) versus DAPI (W) HO (A) versus HO (W)
	405 nm, 50 mW	BP 450/50	DAPI, HO	DAPI (A) HO (A)
	488 nm, 50 mW	LP 620	PI	PI (A)
	561 nm, 50 mW	BP 610/20	PI	PI (A)