Table 2.

HO, DAPI, and PI median fluorescence of gated events of chromosome 18 and Y from a normal male human lymphoblastoid cell line, GM7016A measured using UV and 488 nm lasers

	DAPI/PI						HO/PI
Dye combinations	DAPI median fluorescence			PI median fluorescence			HO median fluorescence			PI median fluorescence
Flow cytometer	18	Y	Ratio (Y/18)	18	Y	Ratio (Y/18)	18	Y	Ratio (Y/18)	18	Y	Ratio (Y/18)
Mo‐Flo	1,458	1,566	1.07	1,455	967	0.66	1,526	1,349	0.88	1,356	951	0.7
BD Influx	18,176	23,824	1.31	19,968	13,600	0.68	18,464	17,184	0.93	18,176	12,784	0.7
BD LSRll	79,552	105,216	1.32	80,000	54,976	0.69	80,000	75,520	0.94	72,832	50,496	0.69
BD LSRFortessa	74,752	99,136	1.33	79,424	54,336	0.68	80,960	76,800	0.95	74,688	52,032	0.70
Average			1.26			0.68			0.93			0.70