Figure 2.

HPV‐negative conditioned medium stimulates markedly increased chemokine release from NTF than HPV‐positive conditioned medium. Chemokine release was quantified by ELISA in the conditioned medium of HPV‐positive (SCC2, SCC90, SCC152) or HPV‐negative (SCC72, SCC89, FaDu) tumour cells cultured alone for (a) CXCL8 (b) CCL2 (c) CCL5. NTF06 were cultured with the conditioned medium produced from either HPV‐positive (SCC2, SCC90, SCC152) or HPV‐negative (SCC72, SCC89, FaDu) tumour cells for 24 h and compared to NTF cultured alone for (d) CXCL8, (e) CCL2 and (f) CCL5. Data are mean ± SD from at least 3 independent experiments performed in triplicate and statistical analysis was achieved using a one‐way independent ANOVA with Tukey's post‐hoc multiple comparison test. *at least p < 0.05 compared to NTF alone; ¶ p < 0.01 compared to SCC2, SCC90 and SCC152. [Color figure can be viewed at wileyonlinelibrary.com]