Figure 1.

Variation in Junbo mouse bulla fluid. (a) The bulla fluid was graded from 1 to 5 on the basis of its appearance, 1 being most serous/clear and 5 being most viscous/opaque (all bars). (b) Average bacterial titers per μl for the respective ear fluid grades post‐7 days intranasal inoculation of NTHi (filled bars). (c) Total cell count for the different grades of ear fluid in noninoculated (empty bars) and inoculated (filled bars) Junbo mice (*P < 0.05, **P < 0.01). (d) Cyto‐spun and giemsa stained images of different grade middle ear fluids (Scale bar: 20 μm)