Figure 2.

T_FH induction precedes germinal centre formation and antibody production. Adult female BALB/c mice were exposed to either 25 μg house dust mite (HDM) or 25 μL phosphate‐buffered saline (PBS), three times a week for up to 5 weeks. A‐B, Flow cytometry was used to determine the frequency of germinal centre (GC) B cells in the mLN and lungs. GC B cell was defined as CD38⁻ GL7⁺ FAS ⁺ CD19⁺B220⁺ lymphocytes and quantified. A, mLN GC B cells, B, Lung GC B cells. C‐D, Serum was titrated, and allergen‐specific IgE and IgG1 were measured by ELISA. Endpoint titres are displayed. C, HDM‐specific IgE, D, HDM‐specific IgG1. Statistical significance was determined using a Mann‐Whitney U test. *P < 0.05, **P < 0.01, ***P < 0.001. n = 5 mice per time‐point. Representative data from two independent experiments