Figure 5.

Therapeutic ICOS‐L blockade reduces pulmonary IL‐13 and IL‐17A. Adult female BALB/c mice were exposed (i.n) to 25 μg house dust mite (HDM) or 25 μL phosphate‐buffered saline (PBS), three times a week for 5 weeks. From the start of week 4, mice were also administered 150 μg anti‐ICOS‐L (α‐ICOS‐L) or isotype control (IgG) antibody (i.p) three times a week. Mice were culled at the end of week 5. Pulmonary cytokines and chemokines within lung homogenate were measured by ELISA. A, IL‐13, B, IL‐17A, C, IL‐21, D, IL‐5, E, Eotaxin‐2. Statistical significance was determined using a Mann‐Whitney U test. *P < 0.05, **P < 0.01, ***P < 0.001. Data are pooled from two independent experiments, n = 8 for PBS‐treated groups, n = 12 for HDM‐treated groups