Figure 1.

Trn shares LRR repeats with the Toll family proteins. A) Diagrams of Drosophila sLRR proteins domains. Diagrams modified from those produced by the SMART program. Most Tolls have large Leucine Rich Repeat regions (LRR, green rectangles), C- (blue ovals) or N- (light blue rectangles) type cysteine rich flanking regions, a transmembrane domain (TM, dark blue rectangles) and an internal Toll/Interleukin-1 Receptor (TIR, green hexagons) domain. Pink squares represent regions of low complexity. Tartan (Trn) and Capricious (Caps) share two of these features, the LRR region and the TM domain, but lack the TIR domain. Chaoptin (Chp), Connectin (Con) and Slit all contain LRR domains and are involved in cell adhesion, but lack the TM and TIR domains. Slit also contains a laminin G domain (LamG, yellow hexagon), an EGF domain (GFP, vertical green pentagons), and a cystein knot-like domain (CT, horizontal green pentagon). B) Simplified phylogenetic tree showing the relationship between Trn and Toll family proteins. For the full tree see Supplemental Figure 1. Orthologs were identified by reciprocal blast of protein sequences, aligned with Clustal Omega and placed in a midpoint rooted RaxML phylogenetic tree using Topali. The clade containing Trn is purple. The clades containing the Tolls that are expressed in a segmental pattern are blue. Numbers at the junctions are bootstrap values from 100 iterations which provide statistical support for the nodes. The size of the triangle is proportional to the number of orthologs within the clade.