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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Physiol Behav. 2018 May 3;194:73–76. doi: 10.1016/j.physbeh.2018.04.033

Table 1.

Details of each corticosterone assay as provided in the manufacturer’s manual.

Abcam Enzo R&D RIA
Range of standard curve 391–100,00 pg/ml 32–20,000 pg/ml 103–25,000 pg/mL 25,000–1,000,000 pg/mL
Sensitivity 8,000 pg/mL 26.99 pg/mL 28 pg/mL 7,700 pg/mL
Dilution method 25-fold 30-fold 50-fold 200-fold
Type of kit Competitive ELISA RIA
Capture method Direct (primary antibody) Sandwich (primary (donkey anti-sheep lgG) and secondary antibodies) Antibody
Detection method Indirect (biotin-streptavidin) Indirect 125I label
Detection chemistry Peroxidase and substrate Alkaline phosphate and substrate HRP and substrate
Sample pretreatment None Incubated for > 5 min with provided steroid displacement reagent Incubated for 15 min with 0.6 N Tricloroacetic acid None
Free or total corticosterone measured Free Total Total Free