Figure 1.

SIRT1 is highly expressed in livers from cholestatic PBC and PSC patients and is induced in response to bile acids in vitro. (A) qPCR analysis of SIRT1 expression in liver samples from healthy individuals (n = 5), PBC (n = 10), and PSC (n = 10) and (B) IHC using an anti‐SIRT1 Ab in liver sections from cholestatic patients compared to healthy donors showing increased gene and protein expression and protein nuclear localization of SIRT1 in hepatocytes and cholangiocytes during cholestasis (original magnification, ×10) with (C) quantification of positively stained nuclei. Healthy individuals, n = 4; PBC, n = 9; PSC, n = 5. (D) qPCR analysis of SIRT1 expression in THLE2 cells cultured for 3 hours with CA, GCA, CDCA, and DCA and at the doses indicated. Values are mean ± SEM; in vitro experiments were performed three times in triplicate; *P < 0.05; **P < 0.01. Abbreviation: Ab, antibody