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. 2019 Jan 29;69(2):699–716. doi: 10.1002/hep.30275

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© 2018 The Authors Hepatology published by Wiley Periodicals, Inc. on behalf of American Association for the Study of Liver Diseases.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Overexpression of SIRT1 correlates with lower presence and activity of FXR and higher accumulation of bile acids in liver. (A) Western blotting of whole‐protein extracts using FXR Ab and further quantification using Image Lab software (Bio‐Rad, Hercules, CA) showing reduced protein presence in SIRT^oe mice. (B) Gene expression analysis of SHP and Cyp7a1 by qPCR in livers 3 and 7 days after BDL. (C) Quantification of bile acid pool size in livers from WT and SIRT mice after BDL by HPLC. (D) qPCR analysis of bile acid transporters after BDL. Values are mean ± SEM; n ≥ 5 animals/time point; *P < 0.05; **P < 0.01 (WT vs. SIRT^oe). Abbreviations: Ab, antibody; HPLC, high‐performance liquid chromatography.