Table 1.
Primers used for carrying out qPCR reactions
| Gene | Sequence | Product size (bp) | T m (°C) |
|---|---|---|---|
| 18s rRNA | F‐5′‐TCGCTAGTTGGCATCGTTTATG‐3′ R‐5′‐CGGAGGTTCGAAGACGATCA‐3′ |
123 | 53 |
| GAPDH | F‐5′‐GTGGAGTCTACTGGTGTCTTC‐3′ R‐5′‐GTGCAGGAGGCATTGCTTACA‐3′ |
1331 | 54 |
| SOD 1 | F‐5′‐AGACCTGGGTAATGTGACCG‐3′ R‐5′‐CGGGCTAAGTGCTTTCAGAG‐3′ |
773 | 59.4 |
| HMOX 1 | F‐5′‐GGAAGAGCTGGACAGAAACG‐3′ R‐5′‐GACAGATCTCCGAGGTAGCG‐3′ |
1413 | 60 |
| GPX 1 | F‐5′‐GAGGCACAACAGTCAGGGAT‐3′ R‐5′‐TCTCCCATAAGGGACACAGG‐3′ |
994 | 59.4 |
Abbreviations: GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; GPX, glutathione peroxidase; HMOX, haem oxigenase; qPCR, quantitative polymerase chain reaction; SOD, superoxide dismutase.