Figure 2.

Partial reversal of MIA‐induced mechanical hypersensitivity in the ipsilateral hind paw of TASTPM mice. A representation of mechanical withdrawal responses in the ipsilateral hind paw of 6 months old TASTPM and age‐ and gender‐matched wild‐type (WT) that were measured at regular intervals prior to (baseline, 0) and up to 28 days post‐intra‐articular administration of MIA (1 mg/10 μL) or saline (control) into the left knee joint (A). Alteration in behaviour compared between experimental groups (MIA vs. Saline) within genotype (WT or TASTPM) (***p < 0.001) and between genotype (TASTPM vs. WT) within experimental group (Saline or MIA) (^## p < 0.01, ^### p < 0.001) was detected using two‐way repeated measures analysis of variance (ANOVA) followed by the Student–Newman–Keuls post hoc test. The number of male and female per experimental group: WT + Saline (n = 10: five males and five females); WT + MIA (n = 8: four males and four females); TASTPM + Saline (n = 8: four males and four females); and TASTPM + MIA (n = 10: five males and five females). As no difference in thresholds was observed, prior to or at regular intervals post‐MIA administration, between male and female in both WT and TASTPM mice, the data were pooled. Data are expressed as mean 50% paw withdrawal threshold (PWT) ± SEM (n = 8–10 mice per experimental group). Area under the curve (AUC) for ipsilateral hind paw was calculated and expressed as allodynia index where a lower value represents more severe mechanical hypersensitivity. All time is the analysis of AUC for baseline – day 28 (B), early phase (baseline – day 7) (C) and late phase (day 10 – day 28) (D). Statistical comparisons between experimental groups within genotype (***p < 0.001) and between genotype within experimental group (^### p < 0.001) were observed using two‐way ANOVA followed by the Student–Newman–Keuls post hoc test. All values are expressed as mean AUC ± SEM (n = 8–10 mice per experimental group).