Figure 3.

A, Normalized osteoblastic ALP activity cultured in gingival fibroblast conditioned media (GFCM) treated with BMP2. B, ROS cell ALP activity following treatment with 10 ng/ml BMP2, 100 ng/ml Noggin, 100 ng/ml Gremlin1 and GFCM. Single dose stimulations employed. C, ROS cell ALP activity stimulated by 10 ng/ml BMP2 is inhibited by 100 ng/ml noggin. Single dose stimulations employed D. 10 ng/ml BMP2 overcomes inhibitory effect on ALP activity of GFCM in ROS cells. Single dose stimulations used. Control in all experiments was standard media. ALP activity assessed after 72 hours and adjusted for cell number in all experiments. E, Inhibitory effect of GFCM on BMP2‐stimulated calvarial osteoblast chemotaxis. Five counts per well. Minimum of three replicates per experiment. Data shown as mean ± SD. Representative data from three independent experiments are shown. Significant differences shown as *P < 0.05 to BMP‐matched control, ^† P < 0.05 to baseline control. Significance tested using one‐way ANOVA with Bonferroni post‐tests