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ELISA analyses of cytokine and chemokine secreted in the supernatant of M2a, M2b, and M2c macrophages polarized in vitro for 72 h. High levels of IL‐10, IL6, TNFα, and CCL1 are secreted by M2b macrophages (white bars), but not by M2a (light gray bars) or M2c macrophages (dark gray bars). The results from three independent experiments were combined for statistical analysis. Bars represent the mean concentration (pg/mL) of the measured cytokines ± STDEV
