Figure 4.

Metabolic activation enhances cAMP‐induced ABCA1 efflux in J774 macrophages. J774 macrophages, labeled with ³H‐cholesterol (1μCi mL^–1) in RPMI media for 24 h were stimulated ± cAMP (0.3 mm) for 1 h and cotreated with glucose, insulin, or palmitic acid (PA) alone or in combination for 18 h in d‐glucose free DMEM. A) Total, B) ABCA1‐dependent, and C) ABCA1‐independent CEC were determined in serum over 4 h (*p < 0.05, **p < 0.01, ***p < 0.001 w.r.t. untreated control (‐glucose, ‐PA, and ‐insulin); ^# p < 0.05 and ^### p < 0.001 w.r.t. 25 mm glucose). D) ABCA1, ABCG1, and SR‐B1 protein levels were determined by immunoblotting (n = 3 in triplicate).