Figure 3.

PSAP/GPR37L1/GPR37–mediated signaling is essential for migration of astrocytes in the scratch assay and the effect of PSAP is mimicked by TX14(A). (a) Astrocytes move into a wound in media containing 5% FBS and in PDM (prosaposin‐depleted media) supplemented with 100 nM TX14(A) but the movement is inhibited in absence of PSAP. Representative images at 0, 24, and 48 hr post scratch. See also Supporting Information Movies SS1–SS3. (b) Dynamics of the relative wound density under conditions shown in a (n = 3, triplicates). (c) Relative wound density 48 hr post scratch for astrocytes incubated in FBS‐containing media, PDM, PDM + TX14(A), PDM + TX14(A) + both knock‐down vectors and PDM + TX14(A) + knock‐down control vector n = 6, triplicates, ***p < .001 vs. indicated group, one‐way ANOVA with Turkey's post hoc analysis). (d): DAPI staining revealed no significant differences in the cell density between astrocytes cultured in media (+FBS), PDM and PDM supplemented with TX14(A) (100 nM; n = 15). (e): The addition of TX14(A) to PDM does not affect the numbers of new astrocytes based on BrdU staining (n = 7, triplicates) [Color figure can be viewed at wileyonlinelibrary.com]