Table 4.
Examples of Lowest Effective Concentrations (LOEC) of Various Model Compounds Found in Micronucleus Experiments with Different Human Derived Cell Types
| Substance/ | Cell type | ||||
|---|---|---|---|---|---|
| Huh6 | HepaRG | HepG2 | Hep3B | Human lymphocytes | |
| AFB1 (μM) | 10.0 | 0.25 (Le Hegarat et al., 2010) | 0.5 (Josse et al., 2012) | 0.5 (Majer et al., 2004) | 0.1 + S9 (Miller et al., 1998) |
| B(a)P (μM) | 5.0 | 5.0 (Le Hegarat et al., 2010) | 25.0 (Josse et al., 2012) 0.5 (Valentin‐Severin et al., 2003) |
25.0 (Majer et al., 2004) | 119.0 + S9 (Miller et al., 1998) 51.0 + S9 (Fowler et al., 2014) |
| PhiP (μM) |
50.0 | Negative (NOEC 320.0) (Le Hegarat et al., 2010) |
25.0 (Majer et al., 2004) | Negative (NOEC 300.0) (Majer et al., 2004) | 2.5 + S9 (Katic et al., 2010) |
| IQ (μM) |
50.0 | Negative (NOEC 500) (Le Hegarat et al., 2010) |
300.0 (Knasmuller et al., 1999) | Not tested | 2200.0 + S9 (Fowler et al., 2014) 250.0 + S9 (Katic et al., 2010) |
| MMS (μg/mL) | 10.0 | 9.9 (Josse et al., 2012) | 11.0 (Valentin‐Severin et al., 2003) | Not tested | 51.0 (Fowler et al., 2014) 15.4 (Andreoli et al., 1999) |
| NDMA (μM) |
1 × 105 | Not tested | 3 × 104 (Majer et al., 2004) 1 × 104 (Valentin‐Severin et al., 2003) |
Negative (NOEC 1.8 × 105) (Majer et al., 2004) | Negative (NOEC 6.75 × 104 + S9; Katic et al., 2010) |
| H2O2
(μM) |
10.0 | Not tested | Not tested | Not tested | 100 (Andreoli et al., 1999) |
| Etop (μg/mL) |
0.1 | 2.5 (Le Hegarat et al., 2014) | 0.1 (Gajski et al., 2016) | Not tested | 0.5 (Fowler et al., 2014) |
| CDDP (μg/mL) |
0.5 | Not tested | 0.1 (Gajski et al., 2016) | Not tested | 1.0 (Fowler et al., 2014) |
S9 – metabolic activation mix; NOEC – highest no observable effect concentration tested.