Figure 4.

Stx2a(cl) and trypsin‐cleaved Stx2a binds to complement FH. Immobilised Stx2a(cl) or Stx2a(uncl) or trypsin‐cleaved Stx2a (2 μg/well each) was incubated with FH (2 μg). As controls trypsin + trypsin inhibitor (PMSF) or BSA were incubated with FH. Additional controls were performed by incubating the proteins with buffer (TBST) instead of FH. As positive control FH was used. The binding capacity of Stx2a(cl) and trypsin‐cleaved Stx2a to FH were significantly higher compared with Stx2a(uncl) and all negative controls (P < 0.001 for all). ***P < 0.001, ****P < 0.0001