Fig. 3.

Cpf1-gRNA catalyzes single-stranded oligonucleotide-mediated site specific mutagenesis in the genome. a Schematic overview of the experimental setup to test for oligonucleotide-mediated mutagenesis. The position of the amber stop-codon is underlined in red. b Strategies to validate the Cpf1 mediate genetic edits in A. nidulans and A. niger. c Efficiency of gene editing at three different sites located in yA and albA. d Sequencing results of five yellow A. nidulans transformants generated by mutagenesis at the yA-gRNA1 target site. Unexpected nucleotide insertion in colony three is indicated in red. HDR homology directed recombination