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. 2019 May 1;6:6. doi: 10.1186/s40694-019-0069-6

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Cpf1-gRNA-mediated gene integration into a specific gene expression site. a Schematic overview of the experimental setup for marker-free specific Cpf1-gRNA-mediated gene insertion. b Plate layout to validate correct gene insertion in A. nidulans and A. niger. c, d visual inspection of A. nidulans and A. niger colonies, respectively, for development of color and production of red fluorescence. IS1 (Integration site 1, see text)