Fig. 3.

Expression of wild-type and mutant Kv1.1 channels. a Cell surface biotinylation of HEK293 cells expressing either mock, Kv1.1 wild type, Kv1.1-p.Leu328Val, or co-expressing Kv1.1 wild type and Kv1.1-p.Leu328Val. Kv1.1 expression was analyzed by immunoblotting for plasma membrane fraction and input from the total cell lysates. Representative immunoblot of 4 independent experiments is shown. b Bar graph representing the quantification of the cell surface biotinylation experiments. It depicts the relative plasma membrane expression compared to input, and is shown as percentage of Kv1.1 wild type. Mean ± SEM is shown.