Figure 1.

TDZD‐8 pretreatment reduced infarct volume 24 hours following neonatal hypoxic‐ischemic brain injury. (A) Timeline of neonatal hypoxic‐ischemic injury and experimental procedures. Seven‐day‐old pups (P7) were randomly selected to be injected with either TDZD‐8 or vehicle solution 20 minutes prior to ischemia induction. This was followed by 90 minutes of recovery and 60 minutes of hypoxia with 7.5% O₂ and 92.5% N₂. TTC staining was performed 24 hours after HI (P8). Neurobehavioral assessment, whole‐brain imaging, Nissl staining, and immunohistochemistry were performed 7 days after the HI (P14). (B) Effect of TDZD‐8 pretreatment on infarct volume. Representative TTC images of coronal brain slices 24 hours after HI injury show brain damage areas in white color (the dotted line on the left of the image represented “mm” in size for each line). (C) Summary of brain infarction volumes of TTC images. Pretreatment with TDZD‐8 significantly reduced the infarct volume compared to the vehicle‐treated group (*P<.05, Student's t test); HI+vehicle group, n=9; HI+TDZD‐8 group, n=20