Figure 4.
Neurotropin® (NTP) improved MMP and affected the expression of HIF‐1α and apoptosis‐related molecules in HT22 cells. (A) Control group. (B) HT22 cells were treated with 40 μmol/L Aβ25‐35 for 24 h. (C) HT22 cells were pretreated with 0.01 UN/mL NTP for 16 h and then coincubated with 40 μmol/L Aβ25‐35 for 24 h. Mitochondrial membrane potential (MMP, △Ψmt) was measured by JC‐1 staining. (F) Statistical results of JC‐1 red/green fluorescence ratio. (E) The protein expression of HIF‐1α, Bcl‐2, and Bax following treatment above. The results were shown as mean±SE from at least three independent experiments. ## P<.01 versus control, *P<.05 and **P<.01 versus Aβ25‐35 group