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Melatonin reduced LPS‐induced oxidative stress in BV2 cells in vitro. (A) The immunostaining images of 8‐OxoG (green) and DAPI (blue) in BV2 cells treated with LPS and melatonin for 4 h. (B) The histogram of ROS assay conducted in BV2 cell lines cultured in 96‐well plates and treated with LPS, melatonin with or without EX527 (SIRT1 inhibitor) for 4 h. The assay was repeated in triplicate. Significance; a,bP < 0.01, 0.05 and y P < 0.05, respectively.
