Figure 4.

CLDN5 decreased the permeability of BBB to A549 cells. The effect of CLDN5 on barrier function of hCMEC/D3 cells to A549 cells was evaluated. Cells invaded were counted under confocal microscope after A549 cells were seeded on the monolayer of hCMEC/D3 cells for 48 hours. (A) Microscope images and quantitative analysis of cells invaded through the monolayer cell in PLL3.7‐CLDN5 and PLL3.7 group, and a significant decrease in cell stained in red(A549) was obtained. (B) The permeability increased in siCLDN5 group, which was manifested by more A549 cells with the membrane stained in red was observed in the lower panel. The A549 cells were stained with CM‐DIL (red), and nucleus was stain for DAPI (blue). Original magnification, 100×. Statistical significance is indicated