Figure 1.

Methamphetamine (METH) decreases grooming time in the splash test and sucrose preference in the sucrose preference test but does not affect anxiety. Effects of a single dose of METH (30 mg/kg, i.p.) on the grooming behavior [splash test (A) and sucrose preference test (B, C)], and on the anxiety‐like behavior [elevated plus maze test (D, E, F)] in mice 3 days after injection. (A) The grooming time, including nose/face grooming (strokes along the snout), head washing (semicircular movements over the top of the head and behind the ears), and body grooming (body fur licking), was significantly decreased in METH mice; (B) sucrose preference was significantly decreased 1 day after METH treatment and returned to basal levels at day 2 post‐METH injection; (C) sucrose consumption was significantly decreased 1 day after METH treatment and significantly increased 2 days after treatment; (D) the percentage of entries in the open arms; (E) the percentage of time spent in open arms; and (F) the number of entries in the closed arms was not statistically different between groups. Animals were monitored during 5 min in the splash test and in the elevated plus maze test. The sucrose preference test was performed during 4 days. Results are mean ± SEM of 7–8 mice in SAL group and eight mice in METH group. *P < 0.05; **P < 0.01; ***P < 0.001 versus saline group using an unpaired Student's t‐test and one‐way ANOVA followed by the Bonferroni post hoc analysis (sucrose preference test) between the indicated experimental groups.