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. 2017 Jan 28;23(4):291–300. doi: 10.1111/cns.12673

Figure 1.

Figure 1

Cryptococcus neoformans exposure initiated upregulation of AnxA2 expression in MBMECs, while the addition of anti‐AnxA2 antibody reduced cryptococcal transcytosis efficiency across endothelial cells. A&B. Quantitative PCR (A) and Western blot (B) assays were performed to evaluate the expression of AnxA2 in MBMECs exposed to C. neoformans at different time points. The above assays were both repeated with similar results. C&D. In vitro transcytosis (C) and association (D; 2 h pi) assay of C. neoformans with MBMECs following treatment with anti‐AnxA2 Ab. Mock IgG was used as a control. ns indicates no statistical significance (P > 0.05). *P < 0.05, **P < 0.01.