Figure 3.

Mechanism of NS383 inhibitory action. A set of intra‐ and extracellular solutions, symmetrical for the primary conduction ion, sodium, was used to obtain a reversal potential of approximately 0 mV. (A) Representative traces of H⁺‐evoked currents (pH 6.5) in an ASIC3 expressing cell at −60 mV and at +60 mV, respectively. After attainment of responses of a repeatable amplitude (only one response shown), 10 μM NS383 or 30 μM amiloride was included in the solutions as indicated by horizontal bars. The interval between stimulations was 30 s. (B) Calculated percent remaining ASIC3 current in the presence of 10 μM NS383 and 30 μM amiloride at −60 mV and +60 mV, respectively. Each bar represents the mean ± SE.