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. 2016 Jun 10;22(9):771–781. doi: 10.1111/cns.12575

Figure 6.

Figure 6

Effect of M1 and M2 microglia on post‐oxygen glucose deprivation (OGD) neuronal survival. Both wild‐type and gld microglia were induced to M1 or M2 phenotype using LPS (100 ng/mL) + IFN‐γ (20 ng/mL) or IL‐4 (20 ng/mL) for 48 h, respectively. The microglial‐conditioned medium were applied over post‐OGD neuron for 6 h. (A) Neuronal cell death was quantified by propidium iodide (red) and calcein AM (green) staining. Scale bar: 50 μm. (B) Mean percentage of neuronal death. (C) lactate dehydrogenase (LDH) release of post‐OGD neuron. (D) Cell viability was quantified by MTT assay. *P < 0.05, **P < 0.01, ***P < 0.001 versus corresponding M0 control group, # P < 0.05, ## P < 0.01 versus WT group. n = 9 per group.