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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Cell Rep. 2018 Oct 2;25(1):95–106.e6. doi: 10.1016/j.celrep.2018.09.002

Figure 1. IFIT1 Depletion in Human Macrophages Leads to an Enhanced LPS-Induced TNF-α Response.

Figure 1.

(A) Schematic for the TNF-α reporter assay used in a genome-wide screen of the LPS response in human THP1 cells (Sun et al., 2017). (B) Screen hit distribution showing TLR4 as a validating positive regulator hit and identification of IFIT1 as a negative regulator of LPS-induced TNF-α expression. (C-E) Primary human macrophages transfected with control or IFIT1 siRNA were stimulated with 100 ng/mL LPS for the indicated times, IFIT1 (C) or TNF (D) mRNA was measured by qPCR, and secreted TNF-α (E) was measured by ELISA. Data are representative of four independent experiments and expressed as mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001 (two-way ANOVA followed by Sidak’s multiple comparison test). See also Figure S1.