(A–C) Control and IFIT1 shRNA-expressing THP1 cells were stimulated with 100 ng/mL LPS for the indicated times, and Sin3A binding was measured by ChIP-qPCR at the (A) IFNB1, (B) IRF7, and (C) IRF8 promoters.
(D–F) Control and IFIT1 shRNA-expressing THP1 cells were stimulated with 100 ng/mL LPS for the indicated times, and IRF3 binding was measured by ChIP-qPCR at the (D) IFNB1, (E) IRF7, and (F) IRF8 promoters.
Data are representative of three independent experiments and are expressed as mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (two-way ANOVA followed by Sidak’s multiple comparison test). See also Figure S5.