KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-IFIT1 | Santa Cruz | Cat# 82946; RRID: AB_2121945 |
| anti-phospho-p38 | Cell Signaling | Cat# 4511S; RRID: AB_2139682 |
| anti-phospho-p65 | Cell Signaling | Cat# 3033S; RRID: AB_331284 |
| anti-p105 | Santa Cruz | Cat# sc293141 |
| anti-GAPDH | Cell Signaling | Cat# 97166 |
| anti-hnRNPL | Santa Cruz | Cat# sc-32317; RRID: AB_627736 |
| anti-phospho-IRF3 | Abcam | Cat# ab76493; RRID: AB_1523836 |
| anti-phospho-JNK | Cell Signaling | Cat# 9255; RRID: AB_2307321 |
| anti-phospho-ERK | Cell Signaling | Cat# 4370; RRID: AB_2315112 |
| anti-phospho-STAT1 | Cell Signaling | Cat# 9167S; RRID: AB_561284 |
| anti-IFIT1 antibody | Novus | Cat# NBP2-33751 |
| anti-FLAG antibody | Sigma | Cat# F7425; RRID: AB_439687 |
| anti-HA antibody | Abcam | Cat# ab130275; RRID: AB_11156884 |
| Anti-rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor®488 Conjugate) | Cell Signaling | Cat# 4412; RRID: AB_1904025 |
| Anti-Mouse IgG (H+L), F(ab’)2 Fragment (Alexa Fluor®488 Conjugate) | Cell Signaling | Cat# 4408; RRID: AB_10694704 |
| anti-Sin3A | Santa Cruz | sc-136318X |
| anti-IRF3 | Santa Cruz | sc-33641X |
| Bacterial and Virus Strains | ||
| Sendai Virus (Cantell) | Gift from Dr. Kathryn Zoon, NIAID, (Bedsaul et al., 2016) | N/A |
| Influenza A (Texas/36/91) | Gift from Dr. Ronald Germain, NIAID, (Brandes et al., 2013) | N/A |
| Burkholderia cenocepacia (J2315) | Gift from Dr. David Greenberg (Al-Khodor et al., 2014) | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| PMA | Sigma | Cat# P8139-5MG |
| GM-CSF | R & D Systems | Cat# 215-GM-010 |
| M-CSF | R & D Systems | Cat# 416-ML-010 |
| LPS from Salmonella minnesota R595 (Re) (TLRgrade) | ENZO Life Sciences | Cat# ALX-581-008-L002 |
| Poly IC | ENZO Life Sciences | Cat# ALX-746-021-M005 |
| Poly(dA:dT) | InvivoGen | Cat# tlrl-patn |
| Leptomycin B | Abcam | Cat# ab120501 |
| Hoechst 33342 | Sigma | Cat# 14533 |
| Recombinant IFN-β | R & D systems | Cat# 11415-1 |
| Deposited Data | ||
| Microarray Data (Gene Expresion Omnibus under) | This Paper | GSE103829 |
| Experimental Models: Cell Lines | ||
| U937 cells | ATCC | Cat# CRL-1593.2 |
| THP1 cells | ATCC | Cat# TIB-202 |
| HEK293T cells | ATCC | Cat# CRL-3216 |
| THP1 B5 (TNF reporter) cells | (Li etal., 2015) | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: Wild type and Ifit1 —/— C57BL/6 mice | Michael Diamond, Washington University, (Szretter et al., 2012) | N/A |
| Oligonucleotides | ||
| IFNB1 F/R primers TCCCAGGAACTCAATGAAGG/ GTGTCGCA ATGGAGTGTTGT |
IDT DNA | N/A |
|
IRF7 F/R primers TTTTTTGAGACTGAGTCTTGCTCT/ CCGCCT GTTCTTATTATTGGAT |
IDT DNA | N/A |
| IRF8 F/R primers ATTTCTCGGAAAGCAGAGCA/ GCCCACTGTGC CTACCTG |
IDT DNA | N/A |
| TNF F/R primers ACACACAAATCAGTCAGTGG/ CTTCTGTCTCGG TTTCTTCTC |
IDT DNA | N/A |
| CCL3 F/R primers TTATAGCAGCTGAGGAAGCAGAA/ TCTCTAA CTCTCAGCTCTCAACTCAT |
IDT DNA | N/A |
| IFIT1 shRNA#1 CCAGACAATGGATATATTAAG | IDT DNA | N/A |
| IFIT1 shRNA#4 GCCCTGAAGCTTCAGGATGAA | IDT DNA | N/A |
| HA IFIT1 F primer GTGTGGTGGAATTCTGCAGATACCATGGGAT CCACCGGTTACCCATACGATGTTCCAGATACGCTCTCGAGATG CCTGATTTAGAAAACAGAGTCTTGG |
IDT DNA | N/A |
| HA IFIT1 reverse primer CCAGAGGTTGATTGTCGAGCGGCCGCC ACTGTGCTGGATCTAAGGACCTTGTCTCACAGAGTTCTCAAAG |
IDT DNA | N/A |
| NLS-HA-F Primer ACCGACTCTAGTCCAGTGTGGTGGAATTCTGC AGATACCATGGGATCCCCTGCAGCAAAACGTGTGAAACTCGAC ACCGGTTACCCATACGATGTTCCAG |
IDT DNA | N/A |
| IFIT1-R primer CCAGAGGTTGATTGTCGAGCGGCCGCCACTGTGC TGGATCTAAGGACCTTGTCTCACAGAGTTCTCAAAG |
IDT DNA | N/A |
| Software and Algorithms | ||
| Statistics: Prism | Graphpad | N/A |
| Other | ||
| HDAC2 siRNA | Dharmacon | D-003495-05 |
| Ifit3 siRNA | Dharmacon | D-040963-03 |
| IFIT1 siRNA1 | Ambion | s7150 |
| IFIT1 siRNA2 | Ambion | s7151 |
| IFIT1 siRNA3 | Ambion | s7152 |
| Non-targeting (NT) siRNA | Dharmacon | D-001210-05-05 |
| TLR4 siRNA | Dharmacon | M-008088-01-0003 |