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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Am J Hematol. 2018 Dec 10;94(2):240–248. doi: 10.1002/ajh.25366

Figure 2: BMP2 is suppressed by LPS and is not required for hepcidin induction by LPS.

Figure 2:

Bmp2fl/fl;Tek-Cre+ and littermate Cre- control male (blue circles, n=6–8 per group) and female (red triangles, n=4–5 per group) mice at 6 weeks of age were injected with PBS or LPS (1 mg per kg body weight) and sacrificed after 6 hours. Livers were collected to determine (A) Il6, (B) Bmp2, (E) Bmp6 and (F) Hamp mRNA levels. Transcripts measured by qRT-PCR were normalized to Rpl19. (C) Liver and (D) serum BMP2 protein levels in Cre- animals were determined by ELISA. Values represent mean ± SEM. *P<0.05, **P<0.01, ***P<0.001 relative to the PBS-treated controls of the same genotype by Student’s t test. Fold-change relative to PBS treated controls of the same genotype as calculated by 2-ΔΔCt are reported in panel F.