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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Am J Hematol. 2018 Dec 10;94(2):240–248. doi: 10.1002/ajh.25366

Figure 4: Neither BMP2 nor BMP6 are regulated by acute serum iron loading, but BMP2 has a partially redundant role, whereas BMP6 is required for hepcidin induction by acute serum iron loading.

Figure 4:

3-week-old male (blue circles) and female (red triangles) Bmp2fl/fl;Tek-Cre+ and littermate Cre- controls (n=13–17 per group, 5–10 of each sex) and Bmp6fl/fl;Tek-Cre+ mice (n=12 per group, 3–4 males and 8–9 females) were weaned to a low iron (2–6 ppm) diet for 3 weeks before treated with 2 mg/kg ferrous sulfate in 0.5 M ascorbic acid or equivalent volume of distilled water by oral gavage. Mice were sacrificed 6 hours after gavage. (A) Serum transferrin saturation and (B) liver iron levels were determined by colorimetric assays. (C) Liver Bmp6, (D) Bmp2 and (G) Hamp mRNA levels were quantified and normalized to Rpl19 by qRT-PCR. (E) Serum and (F) liver BMP2 protein concentrations were measured by ELISA. Values represent mean ± SEM. **P<0.01, ***P<0.001 relative to mice treated with distilled water of the same genotype by Student’s t test. Fold-change relative to vehicle treated controls of the same genotype as calculated by 2-ΔΔCt are reported in panel G.