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. Author manuscript; available in PMC: 2019 Sep 13.
Published in final edited form as: Nature. 2019 Mar 13;567(7748):405–408. doi: 10.1038/s41586-019-1018-5

Figure 3. AmBisome® increased ASL pH in cultured CF airway epithelia and in CFTR−/− pigs.

Figure 3

(a) Concentration-dependent effect of a pre-formed AmB:cholesterol complex on ASL pH (0 μM, n = 37; 0.25 μM, n = 15; 0.5, 50, 100 μM, n = 9; 2 μM, n = 28; 5, 10 μM, n = 16; 20, 25 μM, n = 12) in CuFi-1 (∆F508/∆F508) epithelia. The effect of AmBisome® on (b) ASL pH in CuFi-1 epithelia as a function of time (n = 9) and (c) H14CO3− secretion after 2 h (n = 20). (d) Effect of AmBisome® treatment on ASL pH of CFTR−/− pigs as compared to baseline (n = 4). n are biologically independent samples from at least three independent experiments with similar results. Graphs depict means ± SEM. Panels (a-c): two-sided unpaired Student’s t test (Welch’s correction used for a: 2, 5, 20 μM and b: 60 min). Panel (a), * indicate difference in AmB versus AmB:Chol. Panel (c): * indicate difference versus vehicle. Panel (d): the same pig was measured before and after AmBisome® as indicated by symbols joined by lines; paired Student’s t test. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001. Exact P values in Source Data.