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. Author manuscript; available in PMC: 2019 Sep 13.
Published in final edited form as: Nature. 2019 Mar 13;567(7748):405–408. doi: 10.1038/s41586-019-1018-5

Extended Data Figure 4 |. AmB increases ASL height.

Extended Data Figure 4 |

ASL height, as imaged by confocal microscopy, in (a) NuLi (CFTR+/+) epithelia, (b) CuFi-1 epithelia, (c) CuFi-1 epithelia with apical addition of AmB, (d) CuFi-1 epithelia with apical addition of C35deOAmB, (e) CuFi-1 epithelia with basolateral addition of AmB, and (f) NuLi and (g) AmB-treated CuFi-1 epithelia with basolateral addition of bumetanide (500 μM). Representative images from at least 6 independent experiments are shown. In all panels, measurements were taken from biologically independent samples. Scale bar represents 10 microns.