Extended Data Figure 6 |. AmBisome® increases transepithelial H14CO₃⁻ secretion and ASL pH in a time and dose-dependent manner.

(a) The effect of AmBisome® (1:1000 AmB:lipid ratio), AmB:Chol (1:1000 AmB:lipid ratio in DMSO), and sterile water or DMSO vehicle on H13CO₃⁻ transport across a POPC/10% cholesterol lipid membrane. (b) The effect of AmBisome® (1 mg/mL, 48 hours; n = 16 biologically independent samples, p = 0.0477) or FC-72 vehicle on H14CO₃⁻ movement from the basolateral buffer to the ASL over 10 minutes post-radiolabel addition in CuFi-1 (ΔF508/ΔF508) as normalized to FC-72 vehicle addition. (c) The effect of increasing [AmBisome®] (1 mg/mL, 48 hours; all concentrations, n = 9 biologically independent samples. 0.25 μM p = 0.0106, 2 μM p < 0.0001, 25 μM p = 0.0002, 50 μM p < 0.0001, 100 μM p < 0.0001) on ASL pH in CuFi-1 epithelia as compared to vehicle control. In panel (a), a representative graph from at least three independent experiments is shown. In panel (b), two-sided unpaired Student’s t test was used to assess statistical significance. In panel (c), ANOVA was used to assess statistical significance; delta and statistics are compared to FC-72 vehicle control. Graphs depict means ± SEM; NS, not significant; *P ≤ 0.05; ****P ≤ 0.0001 relative to vehicle control. In (a), the same sample for each replicate was measured repeatedly over time. In (b) and (c), measurements were taken from biologically independent samples.