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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Biochim Biophys Acta Gene Regul Mech. 2019 Mar 2;1862(4):493–508. doi: 10.1016/j.bbagrm.2019.02.008

Figure 4. FOXP1-IT1, a newly cloned novel human long non-coding RNA (lncRNA) enhances human FOXP1 gene promoter activity.

Figure 4.

(A) Schematic Diagram of gene position of FOXP1-IT1 lncRNA embedded within gene of FOXP1 in chromosome 3. (B) Cloned FOXP1-IT1 versions run on 1% agarose gel. Lane 1, FOXP1-IT1, intron spliced; lane 2, FOXP1-IT1int, with intron. (C) Reporter data of co-transfection with FOXP1-IT1 and pNL1.1[Nluc]-P1tata constructs in RAW247.6 cells. (D) Reporter data of co-transfection with FOXP1-IT1int and pNL1.1 [Nluc]-P1tata constructs in RAW247.6 cells. (E) Expression of transfected FOXP1-IT1 and FOXP1-IT1int in RAW264.7 cells as qualitative PCR amplified by splicing-specific primer pair no. 30/ac (Supplementary Table) which crosses the two exons boundary of the lncRNA run on 2% agarose gel. Results are n = 4 separate transfections/nanoluc assays experiments for each of C and D; Data represent the mean ± SD. ***, P<0.001; two tailed T-test.