Figure 3.

DEST data for all four mAb-tag combinations for temperatures from 277 to 313 K. Upper row shows the variation of the visible population and the dark-state population. Lower row shows relaxation rate R₂^dark. The green dashed guidelines show the projected change in R₂ based solely on the effects of temperature and viscosity. Data were obtained in the samples containing equimolar mixtures of labeled mAbs as shown, at total concentrations of 160 mg/mL, with selected data available for 400 mg/mL mixed sample. Error bars are present for all data points, but for some values are smaller than the markers shown. Where P^dark ≈ 0, the value of R₂^dark is not defined, and therefore it is not shown for these points.