Figure 6.

Particle tracking of fluorescently labeled capsular polysaccharide rafts in standard fluorescence microscopy experiments. (a) K1 capsular rafts (labeled with anti-K1 conjugated with antimouse AF647, red) emerge from E. coli cell labeled with GFP (green). (b) Pixelated image used for subpixel tracking. Tracked lyso-PG rafts are shown in yellow. The position of the E. coli outer membrane is shown by the green dotted line. (c) and (d) show the mean square displacement (MSD) as a function of lag time for times of 1 h and 1 h 30 min, respectively; different colors show the results from individual K1 rafts. (e) Subdiffusive power law coefficients, α (from the scaling of the mean square displacement, <r²> ∼ τ^α, where τ is the lag time), with different times after temperature upshifting. The scale bars are 1 μm on (a) and (b).