Figure 4.

Citrullinated vimentin and enolase peptides stimulate CD4 responses in HLA-DP4 transgenic mice. HLA-DP4 transgenic mice were immunised with citrullinated Vim28–49 (ii), Vim415–433 (i), Eno241-260 peptides (iii) or HepB 181–193 (iv) and immune responses specific to the citrullinated or native (wt) peptides were monitored by IFNγ Elispot assay and compared to control (a). Immune responses were assessed in the presence of CD4 or CD8 blocking antibodies (b). Immune responses to the citrullinated human (Hu) Eno241-260 and Vim415-433 peptides were tested for cross-reactivity to the murine (Mo) peptides (c). Immune responses in HLA-DP4 mice were mapped to shorter peptide sequences (d). Results are representative of at least two independent experiments in which n = 3.