Figure 4.
Expression of adenosinergic ectoenzymes on BM MNC upon co-culture with metastatic NB cells.
The expression of CD38, CD203a/PC-1, CD39, and CD73 was evaluated by flow cytometry on BM MNC cultured in the presence (gray bars) or absence (white bars) of metastatic NB cells, at 1:1 MNC:NB ratio. The expression of each molecule was analyzed in CD45+ cells, gating on lymphocytes (panel a), monocytes (panel b) and granulocytes (panel c). Results are expressed as MRFI. p values are indicated where differences are statistically significant (Mann-Whitney test)