Figure 5.

CCR2 antagonist does not reverse the inhibitory effect of CYTL1 on migration. (a, b). CCR2 expression in A549 cells was detected by PCR (a) and FACS (b). (c). Transwell assays of A549 cells treated with RS504393 were performed. Representative images of A549 cell migration obtained from the Transwell assay (magnification × 200) are shown (right), and the cell numbers obtained from the Transwell assays were counted (left). The results are expressed as the means±SEMs from three independent experiments conducted in triplicate. * P < 0.05, ** P < 0.01, and *** P < 0.001 compared with the controls. (d). Effect of CYTL1 (100 ng/ml) on STAT3 phosphorylation in A549 cells treated with SB-431542.